Blot Transfer Systems
Blot Transfer Systems Electroblotting is a technique to immobilise proteins or nucleic acid separation on a solid membrane support. Samples are then detected using specific antibodies, ligands or nucleic acid probes that bind to individual proteins or nucleic acid sequences. This allows identification, quantification or interaction's study of proteins and nucleic acid from various samples, and makes it a powerful technique in proteomics and genomics. Cleaver Scientific offers four types of system: MODULAR ELECTROBLOTTERS – combine PAGE and transfer techniques within the same tank. These options are shown in the PAGE vertical sections TANK TRANSFER SYSTEMS – available with either plate or wire electrodes, support efficient, quantitative transfers over a wide molecular weight range. Plate electrode systems are faster through greater field strength; wire electrodes are more economical, consuming less current and generating less heat. SEMI-DRY TRANSFER SYSTEMS – perfect for rapid, high-intensity transfers of mid-range proteins, 10-100kD in size. MICROFILTRATION (DOT AND SLOT BLOTTING) – does not require electrophoresis and is used to determine the working conditions for a new blotting assay, antibody titres and antibody-antigen specificity. Also suitable for nucleic acids TankBlot Electroblotters Designed primarily for wet electroblotting of proteins, these Electroblotters offer a combination of increased capacity with economy saving features. Both units, Mini 10 x 10cm and Maxi 20 x 20cm, have increased capacity over standard systems with up to five gel blot cassettes utilised at any one time. This is especially useful in high throughput laboratories. A uniform electric field is provided by a high intensity coiled electrode and ensures uniform transfer across the blot surface. The cassette’s open architecture ensures the maximum blot area allows direct transfer of current. Its rigid construction ensures contact between the gel and membrane is retained throughout the blot and an even pressure is maintained. These units are compatible with magnetic stirrers to aid heat dispersal and prevent pH drifts in the buffer due to incomplete buffer mixing. Each system includes a cooling pack to further enhance transfer efficiency by removing excess heat. This also saves on buffer for added economy. KEY FEATURES • Ideal for wet electroblotting of proteins - Western blotting • Up to five gel blot cassettes utilised at any one time • Hinged cassettes for added convenience • Accommodates gel thicknesses from 0.25 up to 3mm TECHNICAL SPECIFICATIONS Unit dimensions Mini 19 x 13 x 19cm (W x D x H) Maxi 24 x 16 x 26cm Max. sample Mini 5 Blots, 10 x 10cm capacity Maxi 5 Blots, 20 x 20cm 20 Blots, 10 x 10cm Buffer volume Mini Min 1000ml; Max 1500ml Maxi Min 4300ml; Max 6000ml double hinged cassettes for added convenience ORDERING INFORMATION EBM10 TankBlot Mini ElectroBlotter, 10 x 10cm System for five cassettes, with tank and lid, 5x cassettes, 12x fibre pads and cooling pack EBM20 TankBlot Maxi ElectroBlotter, 20 x 20cm System for five cassettes, with tank and lid, 5x cassettes, 12x fibre pads and cooling pack SB10C SB10F SB20C SB20F TankBlot Mini Cassette Fibre pads - pk/8 TankBlot Maxi Cassette Fibre pads - pk/6
45 Semi Dry Blotters These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting - typically 15 to 30 minutes. All units can be used for all types of blotting: western, southern and northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. Semi Dry Blotting has the added benefit of economic transfers due to very low buffer volumes – typically only a few millilitres of buffer are required per transfer. The electrodes, comprising platinum coated anode and stainless steel cathode, will exhibit practically no corrosion and so provide many years of trouble free use. Uniform heat dispersion across the blot sandwich ensures stable transfer times and no heat induced sample loss or transfer distortions. KEY FEATURES • Rapid transfer times • Western, Southern and Northern Blots • Economic Transfers due to very low buffer volumes • Screw down lid - accommodates gels from 0.25 up to 10mm • Uniform heat dispersion Dot and Slot Dot Blot and Slot Blot microfiltration manifolds are designed for DNA and RNA filter blot hybridisations and immunological (Ag/Ab) screening applications. Machined from high density acrylic, their precision lapped mating surfaces and leak proof gasket ensures uniform filter contact, preventing lateral transfer of samples– smudging – by ensuring that a complete vacuum is formed. A permanent filter template is provided with each manifold to simplify the cutting of filters to the exact size. A vacuum of approximately 600mm Hg (0.8 Bar) is required during sample application. Four configurations are available for 24 & 48 for slots and 48 & 96 wells for dots in the configuration of standard microplates. Each well is alpha-numerically grid referenced for easy identification. KEY FEATURES • Low cost • • • Easy assembly Alpha-numeric sample identification Four sample configurations Model D48 D96 S24 S48 Configuration 3 x 16 8 x 12 2 x 12 3 x 16 Size of well 6mm diameter 6mm diameter 6 x 0.5mm 6 x 0.5mm 12mm deep 12mm deep 12mm deep 12mm deep Vacuum required ----- 600mg Hg 0.8 BAR with cold trap ----- Unit dimensions 6x9.5x10cm 6x10.5x14cm 6x7.4x8.3cm 6x9.5x10cm Membranes size required 12.1 x 4.4cm 11 x 7.4cm 12.1 x 4.4cm 12.1 x 4.4cm ORDERING INFORMATION SEMI DRY BLOTTERS SD10 Semi Dry Mini, 10 x 10cm System SD20 Semi Dry Midi, 20 x 20cm System DOT & SLOT BLOTTERS CSL-D48 48-well Dot Blot Manifold, 3 x 16 array CSL-D96 96-well Dot Blot Manifold, 8 x 12 array CSL-S24 CSL-S48 24-well Slot Blot Manifold, 2 x 12 array 48-well Slot Blot Manifold, 3 x 16 array www.cleaverscientific.com T_+44 (0)1788 565 300 E_info@cleaverscientific.com
