Aufrufe
vor 5 Jahren

Cleaver Scientific Catalog 2019

  • Text
  • Scientific
  • Laborbedarf
  • Combs
  • Mini
  • Casting
  • Buffer
  • Electrophoresis
  • Gels
  • Multisub
  • Maxi
  • Marker
  • Volume
  • Cleaver
  • Scientific
  • Catalog
Kostengünstiger Elektrophorese-Bedarf von Qualitätshersteller Cleaver Scientific für Life Science!

C O M Ta s s a y COMET

C O M Ta s s a y COMET assay tanks are available in three slide formats to study single cell gel electrophoresis (SCGE), a technique made popular by drug toxicology and carcinogenesis studies for the detection and quantitation of DNA damage in cells. Each tank’s robust construction from ebony acrylic ensures that cells remain free of exposure to background light and DNA damage during electrophoresis, while a cooled central platform provides a convenient surface for slide preparation and control of slide temperature during the assay. Following electrophoresis, DNA damage may be measured using Comet Assay scoring software. KEY FEATURES Quantifying DNA damage and repair in drug development applications and Reproductive science. • Overview: Following genotoxic insult, such as ionizing radiation, the resultant strand breakage of supercoiled duplex DNA reduces the size of the large genomic DNA from which these strands are separated or drawn out by electrophoresis. The genomic DNA then takes on the appearance of a ‘comet’ as its negatively charged broken ends and fragments migrate towards the anode during electrophoresis. • Method: After exposure to a genotoxic insult cells are suspended within low melting point agarose and embedded within a thin layer of agarose on a microscope slide. Cellular protein is then removed by lysis in detergent, when DNA is allowed to unwind in alkaline conditions before electrophoresis. The DNA is electrophoresed, stained and then analysed using fluorescent microscopy and imaging software. For COMET assay cooling we recommend the CSL-CHILLER. This CHILLER is ready assembled with the thermostat mounted on the refrigerator and supplied with insulated tubing and clips to form a system ready to use. A simple-to-use rotor dial plus two keys provide access to the interactive interface for fast, accurate set-up. TECHNICAL SPECIFICATIONS Temperature range -25 to 100°C Stability (water @ 10°C) ± 0.1°C Uniformity (water @ 10°C) ± 0.1°C Setting resolution 0.1°C Display 4 digit LED Timer function 1 min to 99 hrs59 mins Pump flow rate 17 L/min (max.) No. stored temp, values 3 Safety over-temperature adjustable cut-out Heater power 230 V 1.3 kW Height above tank rim 200mm Depth below tank rim 135mm High specification Chiller TECHNICAL SPECIFICATIONS SLIDE CAPACITY 10 20 40 UNIT DIMENSIONS 17X34X9CM 31X34X9CM 33X59X9CM (W X L X H) VOLUME 550ML 1000ML 2100ML This Chilling Plate is custom designed and manufactured specifically for Comet Assay. The Chilling Plate can accommodate 26 Comet assay slides and assists in the Comet Assay process by allowing a rapid solidification of the low melting point agarose on the Comet Assay slides and facilitates easy retrieval of the slides once the agarose gels are solid. ORDERING INFORMATION CSL-COM10 Comet Assay Tank for 10 Slides CSL-COM20 Comet Assay Tank for 20 Slides CSL-COM40 Comet Assay Tank for 40 Slides CSL-CHILLER Chiller unit for active slide temperature control CSL-CHILLPLATE Chill Plate for 26 Comet Assay Slides

c o m PAC - 5 0 Developed in collaboration with the Oxidative Stress Group in the Department of Cancer Studies and Molecular Medicine within the University of Leicester, the COMPAC-50 is a high throughput electrophoresis system, to perform the Comet Assay using a patented vertical slide orientation design. This allows up to 50 slides to be run in a single tank, with a fraction of the footprint of traditional tanks. The unique patented design employs two carriers to hold a total of 50 slides (25 per carrier) vertically. This provides two distinct advantages over conventional Comet Assay systems that utilise a horizontal platform for manual mounting of multiple individual slides. Firstly to produce a highly compact system which saves 75% of Lab space. Secondly by holding 25 slides in a rack this allows slides to be processed together in one batch saving on handling assay time by up to 90%. Consequently, this is not only beneficial for electrophoresis but also in the lysis, neutralisation, staining and washing steps of the Comet Assay, when each batch of slides may be treated during each step respectively using the ten ebony acrylic staining dishes supplied. In addition, the COMPAC-50 benefits from a high performance ceramic cooling base with sliding drawer to accommodate a cool pack, which is frozen before use, to maintain optimal buffer temperature. KEY FEATURES • Patented design used vertical slide orientation to increase throughput • Slide carriers eliminate manual handling decreasing errors and assay time. • Ten staining dishes supplied for batchtreatment of slides during the lysis, neutralisation, staining and washing steps • Ebony acrylic construction ensures reduced exposure to background light and potential DNA damage • Highly compact design optimises electrophoresis efficiency during Comet Assay • Ebony acrylic construction ensures reduced exposure to background light and potential DNA damage • 50 slides may be run within 20 minutes using powerPRO300 power supply (page 62) 51 staining dish slide rack rack in dish draining A B TECHNICAL SPECIFICATIONS Unit Dimensions (WxLxH) 26.5 x 15 x 15cm C D Typical Results Repair of UVB-induced DNA damage in human keratinocytes, using enzyme-modified Comet assay. HaCaT cells were irradiated with 1 J/cm 2 UVB, then allowed to repair in fresh medium and DNA damage analysed at different time points (A) 0 h, (B) 1 h,(C) 6 h, (D) unirradiated (courtesy of Karbaschi, M. University of Leicester, Leicester, UK). Total Slide Capacity 50 slides 25 x 75mm Slide Capacity per Rack 25 Volume 550 ml Recommended Power Supply POWERPRO300 300V, 700MA, 150W ORDERING INFORMATION COMPAC-50 High Throughput Comet System for 50 slides, includes 2x 25 slide carriers, 10x staining dishes, tank with ceramic cooling platform and cool pack, lid and power cables COMPAC-PP300 COMPAC-50 and powerPRO300 Power Supply 300V, 700mA, 150W COMRAC-25 Vertical slide carrier for 25 slides, pk/1 STAINDISH Ebony acrylic stain dish, pk/1 STAINDISH4X Ebony acrylic stain dish, pk/4 COMPAC- 50-PE Positive electrode COMPAC- 50-NE Negative electrode CSL-LMA50 Agarose 50g, Low melting point (Pg 29) www.cleaverscientific.com T_+44 (0)1788 565 300 E_info@cleaverscientific.com

News & Aktionen

Broschüren/Kataloge