CFU reagents & CHEMICALS run SAF runSAFE comprises a stain and loading dye combination to visualise electrophoretic mobility of a wide range of DNA in agarose gels. runSAFE is conveniently supplied in a 6x loading dye which is mixed with 5 parts double-stranded DNA before loading onto an agarose gel. runSAFE is non-toxic, safe for the environment and can be disposed of in the regular laboratory waste without using expensive decontamination methods. runSAFE is sensitive and binds DNA to detect as little as 0.2ng DNA per band within a gel; while gel imaging is best performed using the amber emission filter found on the bluVIEW lid or runDOC filter slide. runSAFE comprises: – general purpose stain for DNA ranging from 50bp markers to large super-coiled plasmid. runSAFE - less DNA damage, improved cloning efficiency Plasmid PCR Products KEY FEATURES • Safe – runSAFE stain has ultra-low toxicity (LC>5000mg/kg) and lacks cell permeability • Convenient – supplied as a ready to use 6x Loading dye; simply add 1 part stain to 5 parts DNA, mix and load your gel • Fast – no time-consuming post-staining or destaining of gels is required. • Sensitive – very low background staining of the gel; detects as little as 0.2ng DNA per band • Flexible – may be used with Blue or UV light 10000 1000 REQUEST A FREE SAMPLE 100 Blue LED System 10 0 15 30 45 60 120 gel cutting tips Slower migrating species, indicative of a linear or relaxed circular vector, resulting from DNA nicking or strand breaks, are significantly reduced in DNA plasmid mixed with run-SAFE and exposed to blue light. The concentration of nicked DNA plasmid increases significantly after 8’ of exposure to EtBR and UV irradiation. Gel Excision Tips offer a convenient and efficient one handed method of removing bands using a simple and rapid two-step process. The tips, in two sizes, 4.0 x 1mm and 6.5 x 1mm, cut directly into agarose or acrylamide gels, so eliminating cross contamination between samples. Alternative methods which require multiple steps including washing or rinsing are slow and tedious. These tips allow a safe and efficient one handed operation, with a push button gel and tip release, providing researchers with uniform extractions. Tips fit standard 1000μl pipettors and are available in bags and racks. ORDERING INFORMATION runSAFE Description Tracking Dyes Size Range CSL-RUNSAFE runSAFE stain, 1ml Bromophenol Blue, Xylene Cyanol FF, Orange G 50bp – 20Kb GEL EXCISION TIPS CSL-GELX4 Rectangular Tips - 4.0mm x 1mm, bag/250 CSL-GELX4 RACK Rectangular Tips - 4.0mm x 1mm, 5x racks of 48 CSL-GELX6.5 Rectangular Tips - 6.5mm x 1mm, bag/250 CSL-GELX6.5RACK Rectangular Tips - 6.5mm x 1mm, 5x racks of 48
eagents & CHEMICALS 27 dnaLADD RS Pre-made and containing loading dye for immediate use, CleaverScientific’s ready-to-use DNA markers are specially formulated to run accurately and produce sharp, well defined ladders. Available in four molecular weight ranges and composed of discrete marker fragments isolated from restriction-digested proprietary plasmids, each DNA marker will remain stable for up to 6 months at room temperature and 12 months if kept in the fridge at 4°C. Each marker contains high intensity reference bands and may be used to perform size comparisons with DNA molecules ranging from the smallest of PCR fragments to large, linearised cosmid vectors. CSL-MDNA-100BPH CSL-MDNA-100BP KEY FEATURES • Ready to use • • • Crisp band patterns Includes bromophenol blue for ease of use Stable at room temperature CSL-MDNA-50BP CSL-MDNA-1KBPLUS REQUEST A FREE SAMPLE Please Note: Ladder banding patterns subject to change, identifiable range will remain the same ORDERING INFORMATION Cat. No. CSL-MDNA-100BPH CSL-MDNA-100BP CSL-MDNA-1KBPLUS CSL-MDNA-50BP Size Range 100-3000bp 100-1500bp 100bp-10kb 50-1500bp Number of bands 12 11 13 17 Reference bands 500, 1500bp 500, 1500bp 1Kb, 3kb 200, 500bp Package concentration 54μg/500μl vial 50μg/500μl vial 50μg/500μl vial 56μg/500μl vial Storage ————————————— 6 months at 25°C, 12 months at 4°C & 24 months at - 20°C ————————————— Recommended loading vol. 5μl/well 5μl/well 5μl/well 5μl/well Tracking dyes Orange G, Xylene Cyanol FF, Bromophenol Blue Source proprietary plasmids and PCR fragments phenol-extracted following restriction digestion and dissolved in 10mM Tris-HCl (pH 8.0) and 10mM EDTA www.cleaverscientific.com T_+44 (0)1788 565 300 E_info@cleaverscientific.com
