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PanReac AppliChem Detergents Aktion Bartelt

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  • Proteins
  • Protein
  • Biology
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  • Biochemica
  • Detergents
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  • Buffer
  • Lysis
  • Solubilization
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Detergents Promotion

PanReac AppliChem Detergents Aktion

IHR LABOR-KOMPLETTAUSSTATTER VERKAUF – GERÄTESERVICE – SOFTWARE bestOffer bestOff This offer is only valid with the indication of the code: APPLIDG Detergents This offer is only valid with the indication of the code: Promotion 22 May -15 Sept 2018 APPLIDG Detergents Promotion -35% on all 22 May -15 Sept 2018 Detergents are also termed as tensides or surfactants. They are soluble in aqueous solution as well as in non-polar organic solvents, and they are able to influence the solubility Detergents are also termed as tensides or surfactants. of other They molecules (like lipids or hydrophobic proteins in buffer solutions). are soluble in aqueous solution as well as in non-polar organic solvents, and they are able to influence the They solubility all have in common that they are soluble amphipathic of other molecules (like lipids or hydrophobic proteins (amphiphilic) in compounds, with both lipophilic (hydrophobic, buffer solutions). nonpolar) and lipophobic (hydrophilic, polar) groups within one molecule. Therefore, they will form stable aggregates They all have in common that they are soluble amphipathic (micelles) above a critical concentration, called CMC value (Critical Micellar Concentration). The CMC value is specific to (amphiphilic) compounds, with both lipophilic (hydrophobic, each detergent and different factors (chemical structure, pH nonpolar) and lipophobic (hydrophilic, polar) groups value, within temperature, ionic strenth) may influence it. one molecule. Therefore, they will form stable aggregates (micelles) above a critical concentration, called CMC Detergents value are widely used in biochemistry, cell biology or (Critical Micellar Concentration). The CMC value is molecular specific to biology. Cell lysis, protein solubilization, protein crystallization or reduction of background staining in each detergent and different factors (chemical structure, pH blotting experiments are just a few of numerous applications. value, temperature, ionic strenth) may influence it. Detergents are widely used in biochemistry, cell biology or molecular biology. Cell lysis, protein solubilization, Examples protein of Applications crystallization or reduction of background staining in blotting experiments are just a few of numerous applications. Purification Examples of Applications Purification • Proteins in Protein Expression, stabilize • Membranes proteins, study of the conformation and • Organelles function of proteins • DNA / RNA, as component of a lysis buffer (lysis of cell nuclei) Solubilization • Membranes • Organelles • Membrane proteins without denaturing them • Proteins in Protein Expression, stabilize proteins, study of the conformation and function of proteins • DNA / RNA, as component of a lysis buffer (lysis of cell nuclei) Solubilization Electrophoresis Blotting (Proteomics and Electrophoresis) • Southern • Western • Northern • ELISA, or other immunostaining • Membrane proteins without denaturing them Electrophoresis • Amino acid and protein separation (SDS-PAGE) • Capillary electrophoresis Chromatography • Stein-Moore (amino acid content analysis) Blotting (Proteomics and Elect • Southern • Western • Northern • ELISA, or other immunostaining • Amino acid and protein separatio • Capillary electrophoresis Chromatography • Stein-Moore (amino acid content

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