LIFE SCIENCES –
LIFE SCIENCES – CHOOSING THE CORRECT ASSAY PLATE CUSTOM MANUFACTURE AUTOMATION TION ACCESSORIES EQUIPMENT SOLID PHASE EXTRACTION STORAGE/COLLECTION 24 Choosing the correct assay plate Absorbance assays – Top Reading Instruments High signal from majority of wells e.g. test kits, ELISA Use a simple solid clear plate Low signal from some or all wells e.g. kinetics or genetic marker assays Use a solid white plate to boost the signal Fluorescence assays – Top Reading Instruments Use a solid black plate Luminescence assays – Top reading instruments Bio- and Chemi- Luminescence use the same plates Good signal Use solid white plates Poor signal or high dynamic range across the plate Use solid Black with White wells An introduction to clear bottomed assay microplates Porvair Krystal series clear bottom plates are now recognised as industry-leading tools for Pharma drug discovery and screening. Our standard clear polystyrene bottom plates have bases just 0.4mm thick (384-well) or 0.75mm (24- and 96-well) and are moulded from highly polished tools to reduce flow lines, artefacts and scratches, guaranteeing good results with all types of bottom-reading instruments. For the more modern demanding confocal readers, Porvair Glass Bottom plates have been shown by leading research institutes to be unsurpassed for flatness, linearity and optical clarity. Our sparkling pure borosilicate glass is just 175µm thick with a flatness tolerance across the plate of +/- 15µm ensuring superb crisp images every time. W W W . M I C R O P L A T E S . C O M Bottom Reading Instruments Normal visualisation: White plates with clear plastic bottoms Confocal visualisation: White plates with glass bottoms For very sensitive assays - Zero Crosstalk white plate with individual clear wells Bottom Reading Instruments Visible wavelength range 350-700nm High signal from majority of wells e.g. GFP, Reporter Gene kits, Use a black clear-bottomed plate Low signal from some or all wells e.g. poor sensitivity assays, low specificity Use a black Zero Crosstalk clear bottom plate to boost the signal-to-noise ratio UV Wavelength range 220-350nm Use black clear-bottomed COP plates Confocal or whole plate imaging: Use black glass-bottomed plates Bottom Reading Instruments Use a white clear-bottomed plate We supply our Krystal clear plates treated for cell culture with a low pressure gas plasma that produces an even surface treatment right across the plate. It is particularly suited to cell lines which do not grow easily on plastics. Our plastic-bottomed plates are ultrasonically welded together – a tried and tested method which both guarantees a perfect seal every time and eliminates the inconsistencies seen with laser membrane welding used by other manufacturers. Only biocompatible adhesives are used for glass bottom plates, cured by UV radiation to ensure that the finished plates are solvent-free, have very low autofluorescence and will allow good cell growth.
LIFE SCIENCES – SOLID BOTTOM ASSAY PLATES 96-well clear plates For routine adsorption, absorption, ELISA, mixing and storage applications the standard range of 96-well assay plates offers the perfect mix of affordability and high quality. l Manufactured from high quality crystal polystyrene l Flat bottom for spectrophotometric work l V-bottom minimizing residual liquid l Round (U) bottom for cell/particulate collection l Robot compatible l Working volumes from 275µl down to 10µl Solid assay plates Plate description Sterile Colour Qty/pack Cat. no. 96 wells of 350µl with a flat bottom – Clear 100 208004 96 wells of 270µl with a round bottom – Clear 100 209004 96 wells of 220µl with a V bottom – Clear 100 210004 W W W . M I C R O P L A T E S . C O M CUSTOM MANUFACTURE AUTOMATION ACCESSORIES EQUIPMENT SOLID PHASE EXTRACTION STORAGE/COLLECTION 25 UIPMENT EQU TION ACCESSORIES OMAT
