Cleaver Scientific Catalog 2019

eagents & CHEMICALS r u

eagents & CHEMICALS r u n S A F The runSAFE range comprises four stain and loading dye combinations to visualize electrophoretic mobility of a wide range of DNA in agarose gels. runSAFE is conveniently supplied in a 6x loading dye which is mixed with 5 parts double-stranded DNA before loading onto an agarose gel. runSAFE is non-toxic, safe for the environment and can be disposed of in the regular laboratory waste without using expensive decontamination methods. runSAFE is sensitive and binds DNA to detect as little as 0.2ng DNA per band within a gel; while gel imaging is best performed using the amber emission filter found on the bluVIEW lid or runDOC filter slide. The runSAFE Range is comprised as follows:: • runSAFE – general purpose stain for DNA ranging from 50bp markers to large super-coiled plasmid • runSAFE-PLUS500 – for DNA larger than 500bp in size • runSAFE-2000 – for DNA vectors and inserts ranging from 500-2000bp • runSAFE-500 – for small DNA fragments, PCR products, sequence tracts and primers less than 500bp runSAFE - less DNA damage, improved cloning efficiency Plasmid • Safe – all four runSAFE stains have ultra-low toxicity (LC>5000mg/kg) and lack cell permeability • Convenient – each stain is supplied as a ready to use 6x Loading dye; simply add 1 part stain to 5 parts DNA, mix and load your gel • Fast – no time-consuming post-staining or de- staining of gels is required. • Sensitive – very low background staining of the gel; detects as little as 0.2ng DNA per band • Flexible – each stain may be used with Blue or UV light PCR Products KEY FEATURES 10000 CFU 1000 100 Blue LED System 10 0 15 30 45 60 120 gel cutting tips Slower migrating species, indicative of a linear or relaxed circular vector, resulting from DNA nicking or strand breaks, are significantly reduced in DNA plasmid mixed with run-SAFE and exposed to blue light. The concentration of nicked DNA plasmid increases significantly after 8’ of exposure to EtBR and UV irradiation. Gel Excision Tips offer a convenient and efficient one handed method of removing bands using a simple and rapid two-step process. The tips, in two sizes, 4.0 x 1mm and 6.5 x 1mm, cut directly into agarose or acrylamide gels, so eliminating cross contamination between samples. Alternative methods which require multiple steps including washing or rinsing are slow and tedious. These tips allow a safe and efficient one handed operation, with a push button gel and tip release, providing researchers with uniform extractions. Tips fit standard 1000µl pipettors and are available in bags and racks. ORDERING INFORMATION runSAFE Description Tracking Dyes Size Range CSL-RUNSAFE runSAFE stain, 1ml Bromophenol Blue, Xylene Cyanol FF, Orange G 50bp – 20Kb CSL-RUNSAFEPLUS500 runSAFE-PLUS500 stain, 1ml Bromophenol Blue, Xylene Cyanol Blue >500bp CSL-RUNSAFE2000 runSAFE- 2000, 1ml Xylene Cyanol Blue, Orange G 500-2000bp CSL-RUNSAFE500 runSAFE-500, 1ml Orange G

eagents & CHEMICALS 27 dnaLADD RS Pre-made and containing loading dye for immediate use, Cleaver Scientific’s ready-to-use DNA markers are specially formulated to run accurately and produce sharp, well defined ladders. Available in six molecular weight ranges and composed of discrete marker fragments isolated from restriction-digested proprietary plasmids, each DNA marker will remain stable for up to 6 months at room temperature and 12 months if kept in the fridge at 4°C. Each marker contains high intensity reference bands and may be used to perform size comparisons with DNA molecules ranging from the smallest of PCR fragments to large, linearised cosmid vectors. CSL-MDNA-100BPH CSL-MDNA-100BP CSL-MDNA-1KB KEY FEATURES • Ready to use • • • Crisp band patterns Includes bromophenol blue for ease of use Stable at room temperature REQUEST A FREE SAMPLE CSL-MDNA-50BP CSL-MDNA-BR CSL-MDNA-HR Please Note: Ladder banding patterns subject to change, identifiable range will remain the same ORDERING INFORMATION Cat. No. CSL-MDNA-100BPH CSL-MDNA-100BP CSL-MDNA-1KB CSL-MDNA-50BP CSL-MDNA-BR CSL-MDNA-HR Size Range 100-3000bp 100-1500bp 250bp-10kb 50-1500bp 100bp-10kb 250bp-25kb Number of bands 12 11 13 17 19 14 Reference bands 500, 1500bp 500, 1500bp 1Kb, 3kb 200, 500bp 500bp, 1.5 & 3kb 1Kb, 3kb Package concentration 54µg/500µl vial 50µg/500µl vial 50µg/500µl vial 56µg/500µl vial 86µg/500µl vial 52µg/500µl vial Storage ––––––––––––– 6 months at 25°C, 12 months at 4°C & 24 months at - 20°C ––––––––––––– Recommended loading vol. 5µl/well 5µl/well 5µl/well 5µl/well 5µl/well 5µl/well Tracking dyes Orange G, Xylene Cyanol FF, Bromophenol Blue Source proprietary plasmids and PCR fragments phenol-extracted following restriction digestion and dissolved in 10mM Tris-HCl (pH 8.0) and 10mM EDTA www.cleaverscientific.com T_+44 (0)1788 565 300 E_info@cleaverscientific.com