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eagents & CHEMICALS

eagents & CHEMICALS buffers&DY S Cleaver Scientific offers a range of concentrated buffers and loading dyes to complement the EZEE multisub horizontal electrophoresis range and to offer a complete solution for the users. These are ideal for laboratories running horizontal nucleic acid gels on a daily basis that require high quality reagents for reproducible results. TBE and TAE buffers Nucleic acid agarose gel electrophoresis is usually conducted with either Tris-acetate-EDTA (TAE) buffer or Tris-borate-EDTA (TBE) buffer. While TAE buffer provides faster electrophoretic migration of linear DNA and better resolution of supercoiled DNA, TBE buffers have a stronger buffering capacity for longer or higher voltage electrophoresis runs. The buffers are available either as ready-to-use stock solutions (50xTAE and 10xTBE) or as dry powder that just need to be reconstitute in distilled water to provide a 10x stock solution (TBE only) 10X Bromophenol Blue DNA loading dye, the standard tracking dye for electrophoresis. The charge-to-mass ratio of bromophenol blue allows it to co-migrate with smaller molecules within agarose and PAGE gels (e.g. at 300bp in a standard 1% agarose, TBE gel) which, with its conspicuous dark blue colour, makes it the perfect tracking dye to monitor the progress of electrophoresis runs. DNA loading dye is supplied in 1ml volumes for easy handling. Powdered and Liquid Buffers Orange G Loading dye 1x (with ficoll) Used as a marker in PAGE and Agarose electrophoresis of DNA, as it migrates through the gel consistently with smaller DNA fragments. Contains sucrose and Xylene Cyanol. Used as a 1x solution. TECHNICAL SPECIFICATIONS TAE FINAL CONSTITUENT CONCENTRATIONS: TRIS ACETATE 0.04M, EDTA 0.001M, PH 8.0 TBE FINAL CONSTITUENT CONCENTRATIONS: TRIS 0.089M, BORIC ACID 0.089M, EDTA 0.002M, PH 8.3 RNAse free water, DEPC-treated to eliminate enzyme activity and then autoclaved, this sterile highly purified water product is perfect for use in PCR and Northern blotting techniques. RNase-Free water is available either as a single 250ml bottle or in fifty 5ml aliquots to prevent cross-contamination. Purified water (18 mega Ohms) for use with sensitive experimental procedures often needs verifying as pyrogen free, this is done using the LAL test or Limulus (Horseshoe crab) amoebocyte lysate assay. The LAL test is extremely sensitive to endotoxins which are the result of bacterial lysis. BP Grade Sterile Water has endotoxins removed by electrostatic filtration at the final purification stage prior to autoclaving. The LAL tested water conforms to the standard having less than 0 WFi compatible. ORDERING INFORMATION POWDERED AND LIQUID BUFFERS CSL-TBEP Powdered Tris-Borate-EDTA Running Buffer, - to make 10x stock/1L – 10 sachets (1 litre / pack) TBE10X1L Buffer Tris-Borate-EDTA Running Buffer, 10 x 1L TBE10X5L TAE50X1L TAE50X5L Buffer Tris-Borate-EDTA Running Buffer, 10 x 5L Buffer Tris-Acetate-EDTA Running Buffer, 50 x 1L Buffer Tris-Acetate-EDTA Running Buffer, 50 x 5L CSL-LOADDYE 10x Bromophenol Blue Loading Dye, 1ml CSL-LOADDYE10 10x Bromophenol Blue Loading Dye, 10ml CSL-ORANGEDYE Orange G Loading Dye, 1ml RFW250 RNase-Free Water, 1x250ml RFW50X5 RNase-Free Water, 50x5ml UPW1000 BP Grade Sterile Water, 1000ml

eagents & CHEMICALS agarOS Cleaver Scientific CleverGEL is an environmentally friendly agarose suitable for analysis of nucleic acids using standard electrophoretic procedures. Available as standard Low EEO, High Resolution PCR grade, Low melting point and Instant agarose tablets. KEY FEATURES CleverGel Low EEO agarose: • Ideal for routine analytical electrophoresis and blotting of DNA and RNA 0.1-10Kb in size • Low EEO • High gel strength 29 CleverGel High Resolution – PCR grade: • High Resolution • Low background for analysis of fragments 20-800bp CleverGEL agarose is suitable for analysis of nucleic acids using standard electrophoretic procedures. It is manufactured by a process which excludes organic solvents harmful to marine life, making them far kinder to the environment than conventional agarose. A low EEO (electroendoosmotic) flow minimises diffusion so that even the smallest of nucleic acid fragments remains sharp and tightly resolved. CleverGEL is available as standard Low EEO, High Resolution PCR grade, Low melting point and Instant agarose tablets. CleverGel Low Melting Point: • Used for nucleic acid recovery • typically resolves fragments 200bp to 25Kb CleverGel Instant Agarose Tablets: • Faster and simpler to prepare • Low EEO • Consistent gel percentage Instant Agarose Tablets TECHNICAL SPECIFICATIONS Low EEO Low Melting Point High Resolution Instant Agarose CAS 9012-36-6 39346-81-1 39346-81-1 9012-36-6 Gelling Point* 36°C±1.5°C 26-30°C ≤33°C 36°C±1.5°C Melting Point* 88°C±1.5°C ≤65°C ≤70°C 88°C±1.5°C Solubility clear, colourless @ 1% [w/v] solution clear, colourless @ 2% [w/v] solution clear, colourless @ 1% [w/v] solution Moisture ≤10% ≤10% ≤10% ≤10% Gel Strength >1200 g/cm 2 (1% [w/v] gel) >200 g/cm 2 (1% [w/v] gel) ≥750 g/cm 2 (1.5% [w/v] gel) >1200 g/cm 2 (1% [w/v] gel) Nuclease & Protease Free yes yes yes yes *For a 1.5% [w/v] gel ORDERING INFORMATION GENERAL PURPOSE CSL-AG5 Agarose 5g, Low EEO CSL-AG100 Agarose 100g, Low EEO CSL-AG500 Agarose 500g, Low EEO CSL-AG1000 Agarose 1000g, Low EEO (2x500g bottles) CSL-AG2000 Agarose 2000g, Low EEO (4x500g) CSL-AG5000 Agarose 5000g, Low EEO (10x500g) CSL-AG10KG Agarose 10Kg, Low EEO (20x500g) LOW MELTING POINT CSL-LMA5 Agarose 5g, LMP CSL-LMA50 Agarose 50g, LMP CSL-LMA100 Agarose 100g, LMP CSL-LMA500 Agarose 500g, LMP HIGH RESOLUTION PCR-GRADE CSL-HRA5 Agarose 5g, HR CSL-HRA100 Agarose 100g, HR CSL-HRA500 Agarose 500g, HR AGAROSE TABLETS CSL-AGTAB Agarose 100g, Low EEO (200x 0.5g tablets, supplied as 20 blister packs of 10x 0.5g tablets) www.cleaverscientific.com T_+44 (0)1788 565 300 E_info@cleaverscientific.com

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