CHROMATOGRAPHY AND ANALYSIS SYRINGES Chromatography and Analysis Syringes In the late 1940’s while working in the radiation lab at UC Berkeley, Clark Hamilton developed various devices, including glove box syringes for handling radioactive material. By the early 1960’s Clark learned about additional liquid handling needs in the field of gas chromatography during a visit to Perkin-Elmer where he set out and developed the first micro syringe for accurate sample injection into GC instruments. In the early 1960’s, two major gas chromatography companies, F&M Scientific and Wilkens Aerograph, approached Hamilton Company to produce high accuracy syringes for the purpose of introducing exact, small volume injections into their GC systems. F&M Scientific and Wilkens Aerograph later became Agilent and Varian, and Hamilton continues to provide syringes for manual and automated injection into their instruments as well as many other GC and HPLC manufacturers. 28
CHROMATOGRAPHY SYRINGES BY SERIES AND | GASTIGHT ANALYSIS SYRINGES Manual HPLC Syringes HPLC syringes are equipped with a point style 3 needle to fit HPLC injection valve seats without damaging the rotor seal. HPLC Autosampler Syringes A wide variety of syringes are offered to fit most HPLC autosamplers on the market. Manual HPLC Syringes P. 30 HPLC Autosampler Syringes P. 33 Manual GC Syringes GC Autosampler Syringes GC syringes are fitted with a point style 2 or point style AS needle to pierce injection port septa without damage. A wide selection of syringes fit most GC autosamplers in the lab. Manual GC Syringes P. 39 GC Autosampler Syringes P. 44 Thin-Layer Chromatography (TLC) Syringes The needle points on Thin- Layer Chromatography (TLC) syringes are coated with a PTFE layer to reduce the surface tension between the needle and the liquid for reproducible sample spotting. Carbon Analyzer Syringes This family of syringes is designed for water analysis in Total Organic Carbon (TOC) analyzers. Thin-Layer Chromatography (TLC) Syringes P. 54 Carbon Analyzer Syringes P. 55 29
