CHROMATOGRAPHY AND ANALYSIS SYRINGES | MANUAL HPLC SYRINGES Manual HPLC Syringes HPLC syringes are specifically designed for manual or automatic injection into high pressure ports. The needle is blunt and electro-polished to slide smoothly into the injection port, reducing the wear on critical seals. The selection of the appropriate syringe for HPLC applications takes the following three aspects into account: the type of injection valve, the sample properties, and the injection volume. The volume of the syringe should be selected based on the volume and type of injection. In partial loop filling, the injection volume should not be greater than half of the loop capacity. In complete loop filling, where the loop size sets the injection volume, the syringe capacity should be greater than twice the loop volume. Rheodyne, Valco, Beckman, and SSI Valves 2.5 µL to 1 mL Constant Rate Syringes 20 µL to 200 µL P. 31 P. 31 Valco VISF-1 Injection Valves 25 µL to 10 mL P. 32 Waters Manual Syringes 10 µL to 250 µL P. 32 30
CHROMATOGRAPHY AND ANALYSIS SYRINGES | MANUAL HPLC SYRINGES Rheodyne, Valco, Beckman, and SSI Valves Syringes for Rheodyne, Valco, Beckman, and SSI Valves. These syringes are available in Cemented Needle (N), Luer Tip Cemented Needle (LTN), and Removable Needle (RN) terminations. For more details on the terminations see page 12. The needles in this section are 51 mm in length and are point style 3. Removable Needle (RN) Cemented Needle (N) P/N Volume Series Gauge 80365 10 µL 700 22s ga 80465 25 µL 700 22s ga 80275 25 µL 1700 22s ga 80565 50 µL 700 22s ga 80975 50 µL 1700 22s ga 80985 50 µL 1700 22 ga 80665 100 µL 700 22s ga 81075 100 µL 1700 22s ga 81085 100 µL 1700 22 ga 80765 250 µL 700 22 ga 80865 500 µL 700 22 ga P/N Volume Series Gauge 87942 2.5 µL 600 22s ga 87943 5 µL 600 22s ga 80065 10 µL 1700 22s ga 80265 25 µL 1700 22s ga 80965 50 µL 1700 22s ga 81065 100 µL 1700 22s ga 81165 250 µL 1700 22 ga 81265 500 µL 1700 22 ga 81365 1 mL 1000 22 ga Luer Tip Cemented Needle (LTN) P/N Volume Series Gauge 81175 250 µL 1700 22s ga 81185 250 µL 1700 22 ga 81216 500 µL 1700 22 ga Constant Rate Syringes The CR700 Constant Rate Syringes have preset volumes that can be dispensed at a constant flow rate. The volume is adjustable with a precisely adjustable micrometer screw. The adjusted volume can be fixed with a locking ring. Reproducible injection speed is obtained by means of a spring. The sample is delivered by pressing the push button. Constant Rate Syringe needles are 51 mm in length and point style 3. P/N Volume Series Gauge 84301 20 µL 1700 22s ga 84303 50 µL 1700 22 ga 84302 200 µL 1700 22 ga 31
